Journal: Cell Regeneration

Article Title: Consistent apparent Young’s modulus of human embryonic stem cells and derived cell types stabilized by substrate stiffness regulation promotes lineage specificity maintenance

doi: 10.1186/s13619-020-00054-4

Figure Lengend Snippet: Dynamic AYM changes of epithelial and mesenchymal cell types on different substrate stiffness. a Epithelial (MDCK, HepaRG, hESCs) and b mesenchymal (LX-2, 3 T3, MSCs) cell types both shown dynamic changes in AYM following passaging and in vitro proliferation. c , d Dynamic changes in AYM in response to different substrate stiffness varies between epithelial and mesenchymal cell types. e Schematic of the pattern of AYM changes in epithelial cells during passaging and proliferation

Article Snippet: MSCs were cultured in mesenchymal stem cell growth medium (BioWit Technologies) according to our previous study (Zeng et al., ).

Techniques: Passaging, In Vitro

Journal: Human Gene Therapy

Article Title: Enhancement of MicroRNA-200c on Osteogenic Differentiation and Bone Regeneration by Targeting Sox2-Mediated Wnt Signaling and Klf4

doi: 10.1089/hum.2019.019

Figure Lengend Snippet: miR-200c involved in mouse craniofacial development and osteogenic differentiation of hBMSCs. (A) μCT images of 7-week-old mice of transgenic PMIS-miR-200c and WT; bar = 1 mm. (B) Quantitative measurement of BV/TV using μCT at different locations of skull of transgenic mice with PMIS-miR-200c compared with WT mice. *p < 0.05, n = 3. (C) Fold change of miR-200c in hBMSCs after osteogenic differentiation. (D) Fold change of ALP, Runx2, and OCN in hBMSCs with endogenous miR-200c inhibited by PMIS 7 days after osteogenic differentiation. *p < 0.05 versus untreated, performed in triplicate. μCT, microcomputed tomography; ALP, alkaline phosphatase; BV/TV, bone volume/tissue volume; hBMSCs, human bone marrow mesenchymal stromal cells; miR, microRNA; OCN, osteocalcin; PMIS, plasmid-based microRNA inhibitor system; Runx2, Runt-related transcription factor 2; WT, wild type. Color images are available online.

Article Snippet: The hBMSCs (ScienCell, Carlsbad, CA) were seeded in six-well plates at a concentration of 10 5 cells per well and cultured with human mesenchymal stem cell growth medium (MSCGM; Lonza, Walkersville, MD).

Techniques: Transgenic Assay, Tomography, Plasmid Preparation

Journal: Human Gene Therapy

Article Title: Enhancement of MicroRNA-200c on Osteogenic Differentiation and Bone Regeneration by Targeting Sox2-Mediated Wnt Signaling and Klf4

doi: 10.1089/hum.2019.019

Figure Lengend Snippet: A graphic summary of the multiple roles of miR-200c in bone formation by mediating Wnt signaling through targeting Sox2 and Klf4. BMPs, bone morphogenetic proteins; IL, interleukin; MSC, mesenchymal stromal cell; NF-κB, nuclear factor-kappa B; TGF, transforming growth factor.

Article Snippet: The hBMSCs (ScienCell, Carlsbad, CA) were seeded in six-well plates at a concentration of 10 5 cells per well and cultured with human mesenchymal stem cell growth medium (MSCGM; Lonza, Walkersville, MD).

Techniques: